![]() ![]() This volume provides the latest information on generating, applying, and analyzing DNA barcodes across the Tree of Life from animals and fungi to protists, algae, and plants. Although DNA barcoding as a methodology has been in use for less than a decade, it has grown exponentially in terms of the number of sequences generated as barcodes as well as its applications. DNA barcoding is a novel concept for taxonomic identification using short, specific genetic markers and has been applied to study a large number of eukaryotes. With the emergence of new sequencing techniques, such as Next-generation sequencing (NGS), ONT MinION nanopore sequencing, and Pac Bio sequencing, DNA barcoding has become more. DNA barcoding utilizes one or more standardized short DNA regions for taxon identification. The process of DNA barcoding entails two basic steps: (1) building the DNA barcode library of known species and (2) matching the barcode sequence of the unknown sample against the barcode library for identification. DNA barcoding is a powerful taxonomic tool to identify and discover species. Taxonomists traditionally make species determinations based on their in-depth. As a biological tool, DNA barcoding is being used to address fundamental ecological and evolutionary questions, such as how species in plant communities are assembled. DNA barcoding adds a level of genetic identification to species classification. As a biodiversity discovery tool, DNA barcoding helps to flag species that are potentially new to science. A simple method of obtaining a DNA barcode is described here. ![]() As a research tool for taxonomists, DNA barcoding assists in identification by expanding the ability to diagnose species by including all life history stages of an organism. The Canadian Centre for DNA Barcoding (CCDB) is the authoritative provider of DNA barcode based species identification services. DNA barcodes are short sequences from a standard region of the mitochondrial cytochrome c oxidase subunit 1 (CO1) gene. DNA barcoding, a new method for the quick identification of any species based on extracting a DNA sequence from a tiny tissue sample of any organism, is now being applied to taxa across the tree of life. ![]()
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